Product Information 

Protein full name :Flag Tag 

Synonyms :DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG 

Immunogen: KLH conjugated Synthetic peptide corresponding to DYKDDDDK 

Isotype : IgG1 

Purity: Affinity purification 

Applications

WB: 1 : 1 0 0 0 ; ICC/IF: 1: 5 0 0 -1: 2 0 0 0 ; IP:1: 50-1: 200 ; ELISA: 1:5000-1:20000; EIA: 1:50000

Storage  

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. 

Storage Buffer 

PBS with 0.02% sodium azide, and 50% glycerol. 

Background  

The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.